In this paper, common pathogenic variants in TPO gene responsible for congenital hypothyroidism have been detected by cost-effective Real-time PCR based method. The heterozygous and homozygous variants of TPO gene are confirmed by the clustering patterns of the curves and differences in melting temperature in High-Resolution Melt Curve Analysis. This study will facilitate screening common mutations in congenital hypothyroidism which are highly prevalent in Bangladesh.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0293570
