High-Resolution Melt Curve Analysis: An Approach for Variant Detection in the TPO Gene of Congenital Hypothyroid Patients in Bangladesh

In this paper, common pathogenic variants in TPO gene responsible for congenital hypothyroidism have been detected by cost-effective Real-time PCR based method. The heterozygous and homozygous variants of TPO gene are confirmed by the clustering patterns of the curves and differences in melting temperature in High-Resolution Melt Curve Analysis. This study will facilitate screening common mutations in congenital hypothyroidism which are highly prevalent in Bangladesh.

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0293570